Real-time 3D single-molecule localization using experimental point spread functions.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 29630062.
- Also identified by DOI 10.1038/nmeth.4661 and PMC identifier 6009849.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
We present a real-time fitter for 3D single-molecule localization microscopy using experimental point spread functions (PSFs) that achieves minimal uncertainty in 3D on any microscope and is compatible with any PSF engineering approach. We used this method to image cellular structures and attained unprecedented image quality for astigmatic PSFs. The fitter compensates for most optical aberrations and makes accurate 3D super-resolution microscopy broadly accessible, even on standard microscopes without dedicated 3D optics.
Medical subject headings
- Imaging, Three-Dimensional
- Single Molecule Imaging