Long-range proton-coupled electron transfer in biological energy conversion: towards mechanistic understanding of respiratory complex I.

Abstract

Biological energy conversion is driven by efficient enzymes that capture, store and transfer protons and electrons across large distances. Recent advances in structural biology have provided atomic-scale blueprints of these types of remarkable molecular machinery, which together with biochemical, biophysical and computational experiments allow us to derive detailed energy transduction mechanisms for the first time. Here, I present one of the most intricate and least understood types of biological energy conversion machinery, the respiratory complex I, and how its redox-driven proton-pump catalyses charge transfer across approximately 300 Å distances. After discussing the functional elements of complex I, a putative mechanistic model for its <i>action-at-a-distance</i> effect is presented, and functional parallels are drawn to other redox- and light-driven ion pumps.