Biobeam-Multiplexed wave-optical simulations of light-sheet microscopy.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 29652879.
- Also identified by DOI 10.1371/journal.pcbi.1006079 and PMC identifier 5898703.
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Abstract
Sample-induced image-degradation remains an intricate wave-optical problem in light-sheet microscopy. Here we present biobeam, an open-source software package that enables simulation of operational light-sheet microscopes by combining data from 105-106 multiplexed and GPU-accelerated point-spread-function calculations. The wave-optical nature of these simulations leads to the faithful reproduction of spatially varying aberrations, diffraction artifacts, geometric image distortions, adaptive optics, and emergent wave-optical phenomena, and renders image-formation in light-sheet microscopy computationally tractable.
Medical subject headings
- Microscopy, Fluorescence
- Software