Ribopeaks: a web tool for bacterial classification through m/z data from ribosomal proteins.
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- Record sourced from PubMed, PMID 29659702.
- Also identified by DOI 10.1093/bioinformatics/bty215.
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Abstract
MALDI-TOF MS is a rapid, sensitive and economic tool for bacterial identification. Highly abundant bacterial proteins are detected by this technique, including ribosomal proteins (r-protein), and the generated mass spectra are compared with a MALDI-TOF MS spectra database. Currently, it allows mainly the classification of clinical bacteria due to the limited number of environmental bacteria included in the spectra database. We present a wide-ranging bacterium classifier tool, called Ribopeaks, which was created based on r-protein data from the Genbank. The Ribopeaks database has more than 28 500 bacterial taxonomic records. It compares the incoming m/z data from MALDI-TOF MS analysis with models stored in the Ribopeaks database created by machine learning and then taxonomically classifies the bacteria. The software is available at http://www.ribopeaks.com. Supplementary data are available at Bioinformatics online.
Medical subject headings
- Bacteria
- Bacterial Proteins
- Ribosomal Proteins