Pushing the resolution limit by correcting the Ewald sphere effect in single-particle Cryo-EM reconstructions.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 29674632.
- Also identified by DOI 10.1038/s41467-018-04051-9 and PMC identifier 5908801.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
The Ewald sphere effect is generally neglected when using the Central Projection Theorem for cryo electron microscopy single-particle reconstructions. This can reduce the resolution of a reconstruction. Here we estimate the attainable resolution and report a "block-based" reconstruction method for extending the resolution limit. We find the Ewald sphere effect limits the resolution of large objects, especially large viruses. After processing two real datasets of large viruses, we show that our procedure can extend the resolution for both datasets and can accommodate the flexibility associated with large protein complexes.