Clonal CD4⁺ T cells in the HIV-1 latent reservoir display a distinct gene profile upon reactivation.

Abstract

Despite suppressive combination antiretroviral therapy (ART), latent HIV-1 proviruses persist in patients. This latent reservoir is established within 48-72 h after infection, has a long half-life^1,2, enables viral rebound when ART is interrupted, and is the major barrier to a cure for HIV-1 ³ . Latent cells are exceedingly rare in blood (∼1 per 1 × 10⁶ CD4⁺ T cells) and are typically enumerated by indirect means, such as viral outgrowth assays^4,5. We report a new strategy to purify and characterize single reactivated latent cells from HIV-1-infected individuals on suppressive ART. Surface expression of viral envelope protein was used to enrich reactivated latent T cells producing HIV RNA, and single-cell analysis was performed to identify intact virus. Reactivated latent cells produce full-length viruses that are identical to those found in viral outgrowth cultures and represent clones of in vivo expanded T cells, as determined by their T cell receptor sequence. Gene-expression analysis revealed that these cells share a transcriptional profile that includes expression of genes implicated in silencing the virus. We conclude that reactivated latent T cells isolated from blood can share a gene-expression program that allows for cell division without activation of the cell death pathways that are normally triggered by HIV-1 replication.