ELISA method to detect α-synuclein oligomers in cell and animal models.
basic_science · Level V
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- Record sourced from PubMed, PMID 29698510.
- Also identified by DOI 10.1371/journal.pone.0196056 and PMC identifier 5919555.
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Abstract
Soluble aggregates of α-synuclein, so-called oligomers, are hypothesized to act as neurotoxic species in Parkinson's disease, Lewy body dementia and multiple systems atrophy, but specific tools to detect these aggregated species are only slowly appearing. We have developed an α-synuclein oligomer ELISA that allows us to detect and compare α-synuclein oligomer levels in different in vivo and in vitro experiments. The ELISA is based on commercially available antibodies and the epitope of the capture antibody MJF14-6-4-2 is folding- and aggregate-dependent and not present on monomers.
Medical subject headings
- Enzyme-Linked Immunosorbent Assay
- Parkinson Disease
- Protein Aggregates
- alpha-Synuclein