Novel Effector Phenotype of Tim-3⁺ Regulatory T Cells Leads to Enhanced Suppressive Function in Head and Neck Cancer Patients.

Abstract

<b>Purpose:</b> Regulatory T (Treg) cells are important suppressive cells among tumor-infiltrating lymphocytes (TIL). Treg cells express the well-known immune checkpoint receptor PD-1, which is reported to mark "exhausted" Treg with lower suppressive function. T-cell immunoglobulin mucin (Tim)-3, a negative regulator of Th1 immunity, is expressed by a sizeable fraction of TIL Tregs, but the functional status of Tim-3⁺ Tregs remains unclear.<b>Experimental Design:</b> CD4⁺CTLA-4⁺CD25^high Treg cells were sorted from freshly excised head and neck squamous cell carcinoma (HNSCC) TIL based on Tim-3 expression. Functional and phenotypic features of these Tim-3⁺ and Tim-3^- TIL Tregs were tested by <i>in vitr</i>o suppression assays and multi-color flow cytometry. Gene-expression profiling and NanoString analysis of Tim-3⁺ TIL Treg were performed. A murine HNSCC tumor model was used to test the effect of anti-PD-1 immunotherapy on Tim-3⁺ Treg.<b>Results:</b> Despite high PD-1 expression, Tim-3⁺ TIL Treg displayed a greater capacity to inhibit naïve T-cell proliferation than Tim-3^- Treg. Tim-3⁺ Treg from human HNSCC TIL also displayed an effector-like phenotype, with more robust expression of CTLA-4, PD-1, CD39, and IFN-γ receptor. Exogenous IFN-γ treatment could partially reverse the suppressive function of Tim-3⁺ TIL Treg. Anti-PD-1 immunotherapy downregulated Tim-3 expression on Tregs isolated from murine HNSCC tumors, and this treatment reversed the suppressive function of HNSCC TIL Tregs.<b>Conclusions:</b> Tim-3⁺ Treg are functionally and phenotypically distinct in HNSCC TIL, and are highly effective at inhibiting T-cell proliferation despite high PD-1 expression. IFN-γ induced by anti-PD-1 immunotherapy may be beneficial by reversing Tim-3⁺ Treg suppression. <i>Clin Cancer Res; 24(18); 4529-38. ©2018 AACR</i>.