Genome-scale engineering of Saccharomyces cerevisiae with single-nucleotide precision.
basic_science · Level V
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- Record sourced from PubMed, PMID 29734295.
- Also identified by DOI 10.1038/nbt.4132.
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Abstract
We developed a CRISPR-Cas9- and homology-directed-repair-assisted genome-scale engineering method named CHAnGE that can rapidly output tens of thousands of specific genetic variants in yeast. More than 98% of target sequences were efficiently edited with an average frequency of 82%. We validate the single-nucleotide resolution genome-editing capability of this technology by creating a genome-wide gene disruption collection and apply our method to improve tolerance to growth inhibitors.
Medical subject headings
- Genetic Engineering
- Saccharomyces cerevisiae