C-BERST: defining subnuclear proteomic landscapes at genomic elements with dCas9-APEX2.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 29735996.
- Also identified by DOI 10.1038/s41592-018-0006-2 and PMC identifier 6202229.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
Mapping proteomic composition at distinct genomic loci in living cells has been a long-standing challenge. Here we report that dCas9-APEX2 biotinylation at genomic elements by restricted spatial tagging (C-BERST) allows the rapid, unbiased mapping of proteomes near defined genomic loci, as demonstrated for telomeres and centromeres. C-BERST enables the high-throughput identification of proteins associated with specific sequences, thereby facilitating annotation of these factors and their roles.
Medical subject headings
- CRISPR-Associated Protein 9
- DNA-(Apurinic or Apyrimidinic Site) Lyase
- Proteomics