Discovery of proteins associated with a predefined genomic locus via dCas9-APEX-mediated proximity labeling.
basic_science · Level V
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- Record sourced from PubMed, PMID 29735997.
- Also identified by DOI 10.1038/s41592-018-0007-1 and PMC identifier 6202184.
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Abstract
Regulation of gene expression is primarily controlled by changes in the proteins that occupy genes' regulatory elements. We developed genomic locus proteomics (GLoPro), in which we combine CRISPR-based genome targeting, proximity labeling, and quantitative proteomics to discover proteins associated with a specific genomic locus in native cellular contexts.
Medical subject headings
- CRISPR-Associated Protein 9
- DNA-(Apurinic or Apyrimidinic Site) Lyase