Single-shot super-resolution total internal reflection fluorescence microscopy.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 29735999.
- Also identified by DOI 10.1038/s41592-018-0004-4 and PMC identifier 7470603.
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Abstract
We combined instant structured illumination microscopy (iSIM) with total internal reflection fluorescence microscopy (TIRFM) in an approach referred to as instant TIRF-SIM, thereby improving the lateral spatial resolution of TIRFM to 115 ± 13 nm without compromising speed, and enabling imaging frame rates up to 100 Hz over hundreds of time points. We applied instant TIRF-SIM to multiple live samples and achieved rapid, high-contrast super-resolution imaging close to the coverslip surface.
Medical subject headings
- Microscopy, Fluorescence