Automated, parallel mass spectrometry imaging and structural identification of lipids.

Ellis, Shane R; Paine, Martin R L; Eijkel, Gert B; Pauling, Josch K; Husen, Peter; Jervelund, Mark W; Hermansson, Martin; Ejsing, Christer S et al. · Nat Methods · 2018

basic_science · Level V

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Abstract

We report a method that enables automated data-dependent acquisition of lipid tandem mass spectrometry data in parallel with a high-resolution mass spectrometry imaging experiment. The method does not increase the total image acquisition time and is combined with automatic structural assignments. This lipidome-per-pixel approach automatically identified and validated 104 unique molecular lipids and their spatial locations from rat cerebellar tissue.

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