CRISPR-FRT targets shared sites in a knock-out collection for off-the-shelf genome editing.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 29884781.
- Also identified by DOI 10.1038/s41467-018-04651-5 and PMC identifier 5993718.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
CRISPR advances genome engineering by directing endonuclease sequence specificity with a guide RNA molecule (gRNA). For precisely targeting a gene for modification, each genetic construct requires a unique gRNA. By generating a gRNA against the flippase recognition target (FRT) site, a common genetic element shared by multiple genetic collections, CRISPR-FRT circumvents this design constraint to provide a broad platform for fast, scarless, off-the-shelf genome engineering.
Medical subject headings
- CRISPR-Cas Systems
- DNA Nucleotidyltransferases
- Gene Editing
- RNA, Guide, CRISPR-Cas Systems