<i>17p12</i> Influences Hematoma Volume and Outcome in Spontaneous Intracerebral Hemorrhage.

Abstract

Hematoma volume is an important determinant of clinical outcome in spontaneous intracerebral hemorrhage (ICH). We performed a genome-wide association study (GWAS) of hematoma volume with the aim of identifying novel biological pathways involved in the pathophysiology of primary brain injury in ICH. We conducted a 2-stage (discovery and replication) case-only genome-wide association study in patients with ICH of European ancestry. We utilized the admission head computed tomography to calculate hematoma volume via semiautomated computer-assisted technique. After quality control and imputation, 7 million genetic variants were available for association testing with ICH volume, which was performed separately in lobar and nonlobar ICH cases using linear regression. Signals with <i>P</i><5×10^-⁸ were pursued in replication and tested for association with admission Glasgow coma scale and 3-month post-ICH dichotomized (0-2 versus 3-6) modified Rankin Scale using ordinal and logistic regression, respectively. The discovery phase included 394 ICH cases (228 lobar and 166 nonlobar) and identified 2 susceptibility loci: a genomic region on 22q13 encompassing <i>PARVB</i> (top single-nucleotide polymorphism rs9614326: β, 1.84; SE, 0.32; <i>P</i>=4.4×10^-8) for lobar ICH volume and an intergenic region overlying numerous copy number variants on <i>17p12</i> (top single-nucleotide polymorphism rs11655160: β, 0.95; SE, 0.17; <i>P</i>=4.3×10^-8) for nonlobar ICH volume. The replication included 240 ICH cases (71 lobar and 169 nonlobar) and corroborated the association for <i>17p12</i> (<i>P</i>=0.04; meta-analysis <i>P</i>=2.5×10^-9; heterogeneity, <i>P</i>=0.16) but not for 22q13 (<i>P</i>=0.49). In multivariable analysis, rs11655160 was also associated with lower admission Glasgow coma scale (odds ratio, 0.17; <i>P</i>=0.004) and increased risk of poor 3-month modified Rankin Scale (odds ratio, 1.94; <i>P</i>=0.045). We identified <i>17p12</i> as a novel susceptibility risk locus for hematoma volume, clinical severity, and functional outcome in nonlobar ICH. Replication in other ethnicities and follow-up translational studies are needed to elucidate the mechanism mediating the observed association.