EASI-tag enables accurate multiplexed and interference-free MS2-based proteome quantification.
basic_science · Level V
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- Record sourced from PubMed, PMID 29915187.
- Also identified by DOI 10.1038/s41592-018-0037-8.
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Abstract
We developed EASI-tag (easily abstractable sulfoxide-based isobaric-tag), a new type of amine-derivatizing and sulfoxide-containing isobaric labeling reagents for highly accurate quantitative proteomics analysis using mass spectrometry. We observed that EASI-tag labels dissociate at low collision energy and generate peptide-coupled, interference-free reporter ions with high yield. Efficient isolation of <sup>12</sup>C precursors and quantification at the MS2 level allowed accurate determination of quantitative differences between up to six multiplexed samples.
Medical subject headings
- Mass Spectrometry