Efficient and precise editing of endogenous transcripts with SNAP-tagged ADARs.
basic_science · Level V
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- Record sourced from PubMed, PMID 29967493.
- Also identified by DOI 10.1038/s41592-018-0017-z and PMC identifier 6322650.
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Abstract
Molecular tools that target RNA at specific sites allow recoding of RNA information and processing. SNAP-tagged deaminases guided by a chemically stabilized guide RNA can edit targeted adenosine to inosine in several endogenous transcripts simultaneously, with high efficiency (up to 90%), high potency, sufficient editing duration, and high precision. We used adenosine deaminases acting on RNA (ADARs) fused to SNAP-tag for the efficient and concurrent editing of two disease-relevant signaling transcripts, KRAS and STAT1. We also demonstrate improved performance compared with that of the recently described Cas13b-ADAR.
Medical subject headings
- Adenosine Deaminase
- RNA Editing
- SNARE Proteins