StaPLs: versatile genetically encoded modules for engineering drug-inducible proteins.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 29967496.
- Also identified by DOI 10.1038/s41592-018-0041-z and PMC identifier 6456726.
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Abstract
Robust approaches for chemogenetic control of protein function would have many biological applications. We developed stabilizable polypeptide linkages (StaPLs) based on hepatitis C virus protease. StaPLs undergo autoproteolysis to cleave proteins by default, whereas protease inhibitors prevent cleavage and preserve protein function. We created StaPLs responsive to different clinically approved drugs to bidirectionally control transcription with zinc-finger-based effectors, and used StaPLs to create single-chain, drug-stabilizable variants of CRISPR-Cas9 and caspase-9.
Medical subject headings
- Gene Expression Regulation
- Protein Engineering