Association between <i>ATP2B1</i> and <i>CACNB2</i> polymorphisms and high blood pressure in a population of Lithuanian children and adolescents: a cross-sectional study.

Abstract

Recently, genome-wide associated studies have identified several genetic loci that are associated with elevated blood pressure and could play a critical role in intracellular calcium homeostasis. The aim of this study was to assess the associations of <i>ATP2B1</i> rs2681472 and <i>CACNB2</i> rs12258967 gene polymorphisms with high blood pressure (HBP) among Lithuanian children and adolescents aged 12-15 years. This was a cross-sectional study of a randomly selected sample of 646 12-15-year-old adolescents who participated in the survey 'The Prevalence and Risk Factors of HBP in 12-15 Year-Old Lithuanian Children and Adolescents (from November 2010 to April 2012)'. Anthropometric parameters and BP were measured. The participants with HBP were screened on two separate occasions. Subjects were genotyped <i>ATP2B1</i> rs2681472 and <i>CACNB2</i> rs12258967 gene polymorphisms using real-time PCR method. The prevalence of HBP was 36.7%, significantly higher for boys than for girls. In the multivariate analysis, after adjustment for body mass index and waist circumference, boys with <i>CACNB2</i> CG genotype, <i>CACNB2</i> GG genotype and <i>CACNB2</i> CG +GG genotype had higher odds of having HBP in codominant (adjusted OR (aOR)=1.92; 95% CI 1.16 to 3.18, p=0.011; and aOR=2.64; 95% CI 1.19 to 5.90, p=0.018) and in dominant (aOR=2.05; 95% CI 1.27 to 3.30, p=0.003) inheritance models. Girls carrying <i>CACNB2</i> CG genotype and <i>CACNB2</i> CG +GG genotype had increased odds of HBP in codominant (aOR=1.82; 95% CI 1.02 to 3.24, p=0.044) and in dominant (aOR=1.89; 95% CI 1.09 to 3.28, p=0.023) inheritance models. Furthermore, significant associations were found in additive models separately for boys (aOR=1.72; 95% CI 1.20 to 2.46, p=0.003) and girls (aOR=1.52; 95% CI 1.05 to 2.20, p=0.027). No significant association was found between <i>ATP2B1</i> gene polymorphism and the odds of HBP. Our results indicate that <i>CACNB2</i> gene polymorphism was significantly associated with higher odds of HBP in Lithuanian adolescents aged 12-15 years.