Genome-wide SWAp-Tag yeast libraries for proteome exploration.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 29988094.
- Also identified by DOI 10.1038/s41592-018-0044-9 and PMC identifier 6076999.
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Abstract
Yeast libraries revolutionized the systematic study of cell biology. To extensively increase the number of such libraries, we used our previously devised SWAp-Tag (SWAT) approach to construct a genome-wide library of ~5,500 strains carrying the SWAT NOP1promoter-GFP module at the N terminus of proteins. In addition, we created six diverse libraries that restored the native regulation, created an overexpression library with a Cherry tag, or enabled protein complementation assays from two fragments of an enzyme or fluorophore. We developed methods utilizing these SWAT collections to systematically characterize the yeast proteome for protein abundance, localization, topology, and interactions.
Medical subject headings
- Genome, Fungal
- Genomic Library
- Proteome
- Saccharomyces cerevisiae