Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 29988095.
- Also identified by DOI 10.1038/s41592-018-0052-9.
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Abstract
Reversibly photoswitchable fluorescent proteins (rsFPs) are gaining popularity as tags for optical nanoscopy because they make it possible to image with lower light doses. However, green rsFPs need violet-blue light for photoswitching, which is potentially phototoxic and highly scattering. We developed new rsFPs based on FusionRed that are reversibly photoswitchable with green-orange light. The rsFusionReds are bright and exhibit rapid photoswitching, thereby enabling nanoscale imaging of living cells.
Medical subject headings
- Luminescent Proteins