An enhanced CRISPR repressor for targeted mammalian gene regulation.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 30013045.
- Also identified by DOI 10.1038/s41592-018-0048-5 and PMC identifier 6129399.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
The RNA-guided endonuclease Cas9 can be converted into a programmable transcriptional repressor, but inefficiencies in target-gene silencing have limited its utility. Here we describe an improved Cas9 repressor based on the C-terminal fusion of a rationally designed bipartite repressor domain, KRAB-MeCP2, to nuclease-dead Cas9. We demonstrate the system's superiority in silencing coding and noncoding genes, simultaneously repressing a series of target genes, improving the results of single and dual guide RNA library screens, and enabling new architectures of synthetic genetic circuits.
Medical subject headings
- CRISPR-Cas Systems
- Gene Expression Regulation
- Gene Silencing