Active PSF shaping and adaptive optics enable volumetric localization microscopy through brain sections.
basic_science · Level V
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- Record sourced from PubMed, PMID 30013047.
- Also identified by DOI 10.1038/s41592-018-0053-8 and PMC identifier 6071422.
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Abstract
Application of single-molecule switching nanoscopy (SMSN) beyond the coverslip surface poses substantial challenges due to sample-induced aberrations that distort and blur single-molecule emission patterns. We combined active shaping of point spread functions and efficient adaptive optics to enable robust 3D-SMSN imaging within tissues. This development allowed us to image through 30-μm-thick brain sections to visualize and reconstruct the morphology and the nanoscale details of amyloid-β filaments in a mouse model of Alzheimer's disease.
Medical subject headings
- Brain
- Single Molecule Imaging