A Rho signaling network links microtubules to PKD controlled carrier transport to focal adhesions.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 30028295.
- Also identified by DOI 10.7554/eLife.35907 and PMC identifier 6070338.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Protein kinase D (PKD) is a family of serine/threonine kinases that is required for the structural integrity and function of the Golgi complex. Despite its importance in the regulation of Golgi function, the molecular mechanisms regulating PKD activity are still incompletely understood. Using the genetically encoded PKD activity reporter G-PKDrep we now uncover a Rho signaling network comprising GEF-H1, the RhoGAP DLC3, and the Rho effector PLCε that regulate the activation of PKD at trans-Golgi membranes. We further show that this molecular network coordinates the formation of TGN-derived Rab6-positive transport carriers delivering cargo for localized exocytosis at focal adhesions.
Medical subject headings
- Focal Adhesions
- Microtubules
- Protein Kinase C
- Signal Transduction
- trans-Golgi Network