Genetic dissection of clonal lineage relationships with hydroxytamoxifen liposomes.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 30061668.
- Also identified by DOI 10.1038/s41467-018-05436-6 and PMC identifier 6065311.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Targeted genetic dissection of tissues to identify precise cell populations has vast biological and therapeutic applications. Here we develop an approach, through the packaging and delivery of 4-hydroxytamoxifen liposomes (LiTMX), that enables localized induction of CreER<sup>T2</sup> recombinase in mice. Our method permits precise, in vivo, tissue-specific clonal analysis with both spatial and temporal control. This technology is effective using mice with both specific and ubiquitous Cre drivers in a variety of tissue types, under conditions of homeostasis and post-injury repair, and is highly efficient for lineage tracing and genetic analysis. This methodology is directly and immediately applicable to the developmental biology, stem cell biology and regenerative medicine, and cancer biology fields.
Medical subject headings
- Cell Lineage
- Liposomes
- Tamoxifen