Optimization of fluorophores for chemical tagging and immunohistochemistry of Drosophila neurons.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 30110347.
- Also identified by DOI 10.1371/journal.pone.0200759 and PMC identifier 6093644.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
The use of genetically encoded 'self-labeling tags' with chemical fluorophore ligands enables rapid labeling of specific cells in neural tissue. To improve the chemical tagging of neurons, we synthesized and evaluated new fluorophore ligands based on Cy, Janelia Fluor, Alexa Fluor, and ATTO dyes and tested these with recently improved Drosophila melanogaster transgenes. We found that tissue clearing and mounting in DPX substantially improves signal quality when combined with specific non-cyanine fluorophores. We compared and combined this labeling technique with standard immunohistochemistry in the Drosophila brain.
Medical subject headings
- Drosophila melanogaster
- Fluorescent Dyes
- Immunohistochemistry
- Neurons
- Staining and Labeling