A mechanism for preventing asymmetric histone segregation onto replicating DNA strands.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 30115745.
- Also identified by DOI 10.1126/science.aat8849 and PMC identifier 6597248.
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Abstract
How parental histone (H3-H4)<sub>2</sub> tetramers, the primary carriers of epigenetic modifications, are transferred onto leading and lagging strands of DNA replication forks for epigenetic inheritance remains elusive. Here we show that parental (H3-H4)<sub>2</sub> tetramers are assembled into nucleosomes onto both leading and lagging strands, with a slight preference for lagging strands. The lagging-strand preference increases markedly in budding yeast cells lacking Dpb3 and Dpb4, two subunits of the leading strand DNA polymerase, Pol ε, owing to the impairment of parental (H3-H4)<sub>2</sub> transfer to leading strands. Dpb3-Dpb4 binds H3-H4 in vitro and participates in the inheritance of heterochromatin. These results indicate that different proteins facilitate the transfer of parental (H3-H4)<sub>2</sub> onto leading versus lagging strands and that Dbp3-Dpb4 plays an important role in this poorly understood process.
Medical subject headings
- DNA Replication
- Epigenesis, Genetic
- Histones
- Saccharomycetales