Genetic regulation of mitotic competence in G<sub>0</sub> quiescent cells.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 30116786.
- Also identified by DOI 10.1126/sciadv.aat5685 and PMC identifier 6093628.
- Licence recorded as CC BY-NC.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
Quiescent (G<sub>0</sub> phase) cells must maintain mitotic competence (MC) to restart the cell cycle. This is essential for reproduction in unicellular organisms and also for development and cell replacement in higher organisms. Recently, suppression of MC has gained attention as a possible therapeutic strategy for cancer. Using a <i>Schizosaccharomyces pombe</i> deletion-mutant library, we identified 85 genes required to maintain MC during the G<sub>0</sub> phase induced by nitrogen deprivation. G<sub>0</sub> cells must recycle proteins and RNA, governed by anabolism, catabolism, transport, and availability of small molecules such as antioxidants. Protein phosphatases are also essential to maintain MC. In particular, Nem1-Spo7 protects the nucleus from autophagy by regulating Ned1, a lipin. These genes, designated GZE (G-Zero Essential) genes, reveal the landscape of genetic regulation of MC.
Medical subject headings
- Autophagy
- Cell Nucleus
- Gene Expression Regulation, Fungal
- Mitosis
- Resting Phase, Cell Cycle
- Schizosaccharomyces
- Schizosaccharomyces pombe Proteins