Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 30125268.
- Also identified by DOI 10.1038/nbt.4198.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
Base editors (BEs) enable the generation of targeted single-nucleotide mutations, but currently used rat APOBEC1-based BEs are relatively inefficient in editing cytosines in highly methylated regions or in GpC contexts. By screening a variety of APOBEC and AID deaminases, we show that human APOBEC3A-conjugated BEs and versions we engineered to have narrower editing windows can mediate efficient C-to-T base editing in regions with high methylation levels and GpC dinucleotide content.
Medical subject headings
- CRISPR-Associated Protein 9
- Cytidine Deaminase
- Gene Editing
- Proteins