Modified aptamers enable quantitative sub-10-nm cellular DNA-PAINT imaging.
basic_science · Level V
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- Record sourced from PubMed, PMID 30127504.
- Also identified by DOI 10.1038/s41592-018-0105-0 and PMC identifier 6345375.
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Abstract
Although current implementations of super-resolution microscopy are technically approaching true molecular-scale resolution, this has not translated to imaging of biological specimens, because of the large size of conventional affinity reagents. Here we introduce slow off-rate modified aptamers (SOMAmers) as small and specific labeling reagents for use with DNA points accumulation in nanoscale topography (DNA-PAINT). To demonstrate the achievable resolution, specificity, and multiplexing capability of SOMAmers, we labeled and imaged both transmembrane and intracellular targets in fixed and live cells.
Medical subject headings
- Aptamers, Nucleotide