ESCRT-III mediates budding across the inner nuclear membrane and regulates its integrity.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 30139939.
- Also identified by DOI 10.1038/s41467-018-05889-9 and PMC identifier 6107581.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Vesicle-mediated nucleocytoplasmic transport is a nuclear pore-independent mechanism for the nuclear export of macromolecular complexes, but the molecular basis for this transport remains largely unknown. Here we show that endosomal sorting complex required for transport-III (ESCRT-III) is recruited to the inner nuclear membrane (INM) during the nuclear export of herpes simplex virus 1 (HSV-1). Scission during HSV-1 budding through the INM is prevented by depletion of ESCRT-III proteins. Interestingly, in uninfected human cells, the depletion of ESCRT-III proteins induces aberrant INM proliferation. Our results show that HSV-1 expropriates the ESCRT-III machinery in infected cells for scission of the INM to produce vesicles containing progeny virus nucleocapsids. In uninfected cells, ESCRT-III regulates INM integrity by downregulating excess INM.
Medical subject headings
- Active Transport, Cell Nucleus
- Endosomal Sorting Complexes Required for Transport
- Herpesvirus 1, Human
- Nuclear Envelope
- Virus Release