Characterization of the Proliferating Layer Chondrocytes of Growth Plate for Cartilage Regeneration.

Liu, Zi-Miao; Shen, Po-Chih; Lu, Cheng-Chang; Chou, Shih-Hsiang; Tien, Yin-Chun · Tissue Eng Part A · 2019

basic_science · Level V

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Abstract

In recent years, cell-based therapy is a promising strategy for repairing defect cartilage. However, <i>in vitro</i> expansion of articular chondrocytes (ACs) for collecting enough cell numbers eventually develops cell de-differentiation. In the present study, we choose the proliferative layer chondroctytes (PLCs) of growth plate as new candidate. The novel findings include (1) the higher proliferation potential of PLCs in comparison with the ACs, (2) PLCs produced more GAG than ACs, (3) the increased in GAG matrix production, (4) and lower senescence in PLCs. From these results, we found PLCs might be suitable as cell source for cartilage regeneration.

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