Trac-looping measures genome structure and chromatin accessibility.
basic_science · Level V
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- Record sourced from PubMed, PMID 30150754.
- Also identified by DOI 10.1038/s41592-018-0107-y and PMC identifier 7212307.
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Abstract
Long-range chromatin interactions play critical roles in genome organization and regulation of transcription. We now report transposase-mediated analysis of chromatin looping (Trac-looping) for simultaneous detection of multiscale genome-wide chromatin interactions among regulatory elements and chromatin accessibility. With this technique, a bivalent oligonucleotide linker is inserted between two interacting regions such that the chromatin interactions are captured without prior chromatin fragmentation and proximity-based ligation. Application of Trac-looping to human CD4<sup>+</sup> T cells revealed substantial reorganization of enhancer-promoter interactions associated with changes in gene expression after T cell receptor stimulation.
Medical subject headings
- Chromatin
- Genome, Human