The Congo red derivative FSB binds to curli amyloid fibers and specifically stains curliated E. coli.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 30161215.
- Also identified by DOI 10.1371/journal.pone.0203226 and PMC identifier 6117054.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
The Congo red derivative (E,E)-1-fluoro-2,5-bis(3-hydroxycarbonyl-4-hydroxy) styrylbenzene (FSB) specifically stains the functional amyloid curli in Escherichia coli biofilms. FSB binds to curli with similar affinity as Congo red, yet exhibits much greater fluorescence upon binding to curli as compared to Congo red and does not exhibit undesired binding to the cellulosic component of the biofilm. Thus, FSB presents a powerful tool to identify and visualize curli in E. coli biofilms and also enables new biophysical investigations of curli.
Medical subject headings
- Amyloid
- Bacterial Proteins
- Escherichia coli
- Fluorescent Dyes
- Styrenes