Comment on "Innovative scattering analysis shows that hydrophobic disordered proteins are expanded in water".
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- Record sourced from PubMed, PMID 30166459.
- Also identified by DOI 10.1126/science.aar7101 and PMC identifier 7507683.
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Abstract
Riback <i>et al</i> (Reports, 13 October 2017, p. 238) used small-angle x-ray scattering (SAXS) experiments to infer a degree of compaction for unfolded proteins in water versus chemical denaturant that is highly consistent with the results from Förster resonance energy transfer (FRET) experiments. There is thus no "contradiction" between the two methods, nor evidence to support their claim that commonly used FRET fluorophores cause protein compaction.
Medical subject headings
- Protein Conformation
- Scattering, Small Angle