Experimental Woodsmoke Exposure During Exercise and Blood Oxidative Stress.

Peters, Bridget; Ballmann, Christopher; Quindry, Tiffany; Zehner, Emily G; McCroskey, Justin; Ferguson, Matthew; Ward, Tony; Dumke, Charles et al. · J Occup Environ Med · 2018

basic_science · Level V

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Abstract

The current laboratory study quantified blood oxidative stress to woodsmoke exposure. Participants inhaled woodsmoke during three randomized crossover exercise trials (Clean Air [0 μg/m], Low Exposure [250 μg/m], and High Exposure [500 μg/m], Woodsmoke [particulate matter less than 2.5 μm, PM2.5]). Trolox equivalent antioxidant capacity (TEAC), uric acid (UA), 8-isoprostanes (8-ISO), lipid hydroperoxides (LOOH), protein carbonyls (PC), nitrotyrosine (3-NT), 8-isoprostane, and myeloperoxidase (MPO) were quantified in Pre, immediately Post, and 1- (1Hr) hour post blood samples. UA decreased following Low Exposure, while plasma TEAC levels increased Post and 1Hr. LOOH levels decreased 1Hr Post (High Exposure), while 8-Iso increased following both smoke trials. PC and MPO were unchanged following all trials, while 3-NT increased over Clean Air. Blood oxidative stress occurred largely independent of PM2.5 concentrations. Future studies should employ longer duration smoke and exercise combined with physiologic parameters.

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