Discovery of widespread type I and type V CRISPR-Cas inhibitors.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 30190308.
- Also identified by DOI 10.1126/science.aau5174 and PMC identifier 6520112.
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Abstract
Bacterial CRISPR-Cas systems protect their host from bacteriophages and other mobile genetic elements. Mobile elements, in turn, encode various anti-CRISPR (Acr) proteins to inhibit the immune function of CRISPR-Cas. To date, Acr proteins have been discovered for type I (subtypes I-D, I-E, and I-F) and type II (II-A and II-C) but not other CRISPR systems. Here, we report the discovery of 12 <i>acr</i> genes, including inhibitors of type V-A and I-C CRISPR systems. AcrVA1 inhibits a broad spectrum of Cas12a (Cpf1) orthologs-including MbCas12a, Mb3Cas12a, AsCas12a, and LbCas12a-when assayed in human cells. The <i>acr</i> genes reported here provide useful biotechnological tools and mark the discovery of <i>acr</i> loci in many bacteria and phages.
Medical subject headings
- Bacterial Proteins
- CRISPR-Cas Systems
- Endonucleases
- Gene Editing
- Moraxella
- Pseudomonas