COMRADES determines in vivo RNA structures and interactions.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 30202058.
- Also identified by DOI 10.1038/s41592-018-0121-0 and PMC identifier 6168409.
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Abstract
The structural flexibility of RNA underlies fundamental biological processes, but there are no methods for exploring the multiple conformations adopted by RNAs in vivo. We developed cross-linking of matched RNAs and deep sequencing (COMRADES) for in-depth RNA conformation capture, and a pipeline for the retrieval of RNA structural ensembles. Using COMRADES, we determined the architecture of the Zika virus RNA genome inside cells, and identified multiple site-specific interactions with human noncoding RNAs.
Medical subject headings
- High-Throughput Nucleotide Sequencing
- Nucleic Acid Conformation
- RNA, Viral
- RNA-Binding Proteins
- Zika Virus
- Zika Virus Infection