Rapid functional genetics of the oligodendrocyte lineage using pluripotent stem cells.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 30213958.
- Also identified by DOI 10.1038/s41467-018-06102-7 and PMC identifier 6137209.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Oligodendrocyte dysfunction underlies many neurological disorders, but rapid assessment of mutation-specific effects in these cells has been impractical. To enable functional genetics in oligodendrocytes, here we report a highly efficient method for generating oligodendrocytes and their progenitors from mouse embryonic and induced pluripotent stem cells, independent of mouse strain or mutational status. We demonstrate that this approach, when combined with genome engineering, provides a powerful platform for the expeditious study of genotype-phenotype relationships in oligodendrocytes.
Medical subject headings
- Cell Lineage
- Oligodendroglia
- Pluripotent Stem Cells