Therapeutic Effect of a Xeno-Free Three-Dimensional Stem Cell Mass in a Hind Limb Ischemia Model.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 30215295.
- Also identified by DOI 10.1089/ten.TEA.2018.0089.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
We describe an innovative method of culturing a three-dimensional cell mass (3DCM) of human adipose-derived stem cells (hASCs) in the xeno-free (XF) condition for the treatment of severe ischemic diseases. The majority of the mice injected with XF-3DCMs exhibited limb salvaging and displayed similar blood perfusion compared to normal limbs. <i>In vivo</i> tumorigenicity and toxicity analysis showed that XF-3DCMs did not transform into tumor cells and induce toxicity, respectively. Our results strongly suggest that XF-3DCMs can be effectively used for therapeutic applications and eliminate immunological reaction of animal-derived material contamination.
Medical subject headings
- Hindlimb
- Ischemia
- Stem Cell Transplantation
- Stem Cells