Template-free 2D particle fusion in localization microscopy.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 30224671.
- Also identified by DOI 10.1038/s41592-018-0136-6.
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Abstract
Methods that fuse multiple localization microscopy images of a single structure can improve signal-to-noise ratio and resolution, but they generally suffer from template bias or sensitivity to registration errors. We present a template-free particle-fusion approach based on an all-to-all registration that provides robustness against individual misregistrations and underlabeling. We achieved 3.3-nm Fourier ring correlation (FRC) image resolution by fusing 383 DNA origami nanostructures with 80% labeling density, and 5.0-nm resolution for structures with 30% labeling density.
Medical subject headings
- DNA
- Image Processing, Computer-Assisted
- Microscopy, Fluorescence
- Nanostructures
- Single Molecule Imaging