Association of MTHFR and TNF-α genes polymorphisms with susceptibility to Legg-Calve-Perthes disease in Iranian children: A case-control study.

Azarpira, Mohammad Reza; Ghilian, Mohammad Mahdi; Sobhan, Mohammad Reza; Mehdinezhad-Yazdi, Masoud; Aghili, Kazem; Miresmaeili, Seyed Mohsen; Neamatzadeh, Hossein · J Orthop · 2018

case_control · Level III

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Abstract

The aim of this study was to assess the association of MTHFR and TNF-α genes polymorphisms with Legg-Calvé-Perthes' disease (LCPD) risk in the Iranian children. A total of 45 children with LCPD and 55 healthy controls were recruited to the study. Genotyping was performed via the RFLP-PCR method and genetic risk was calculated by odds ratio (OR) with its corresponding 95% confidence interval (CI). Our case-control study failed to determine any association of MTHFR (677C > T and 1298A > C) and TNF-α (-308G > A and -238G > A) polymorphisms with LCPD risk. More studies with larger sample size are warranted to validate our findings.