Robust single-cell DNA methylome profiling with snmC-seq2.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 30237449.
- Also identified by DOI 10.1038/s41467-018-06355-2 and PMC identifier 6147798.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Single-cell DNA methylome profiling has enabled the study of epigenomic heterogeneity in complex tissues and during cellular reprogramming. However, broader applications of the method have been impeded by the modest quality of sequencing libraries. Here we report snmC-seq2, which provides improved read mapping, reduced artifactual reads, enhanced throughput, as well as increased library complexity and coverage uniformity compared to snmC-seq. snmC-seq2 is an efficient strategy suited for large-scale single-cell epigenomic studies.
Medical subject headings
- DNA Methylation
- Sequence Analysis, DNA
- Single-Cell Analysis