Methodological Issues in Studying PAHSA Biology: Masking PAHSA Effects.

Syed, Ismail; Lee, Jennifer; Peroni, Odile D; Yore, Mark M; Moraes-Vieira, Pedro M; Santoro, Anna; Wellenstein, Kerry; Smith, Ulf et al. · Cell Metab · 2018

editorial · Level V

Where this comes from

Abstract

PAHSAs are anti-diabetic and anti-inflammatory lipids. Syed et al. identify numerous experimental differences that likely account for the failure of Pflimlin et al. to observe PAHSA beneficial effects. The differences include different HFDs resulting in minimal/no glucose intolerance, different assay conditions, an LC-MS protocol that was not validated, and use of olive oil, a bioactive nutrient that improves glucose tolerance, as a vehicle.

Medical subject headings