Reducing effects of particle adsorption to the air-water interface in cryo-EM.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 30250056.
- Also identified by DOI 10.1038/s41592-018-0139-3 and PMC identifier 6168394.
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Abstract
Most protein particles prepared in vitreous ice for single-particle cryo-electron microscopy (cryo-EM) are adsorbed to air-water or substrate-water interfaces, which can cause the particles to adopt preferred orientations. By using a rapid plunge-freezing robot and nanowire grids, we were able to reduce some of the deleterious effects of the air-water interface by decreasing the dwell time of particles in thin liquid films. We demonstrated this by using single-particle cryo-EM and cryo-electron tomography (cryo-ET) to examine hemagglutinin, insulin receptor complex, and apoferritin.
Medical subject headings
- Air
- Apoferritins
- Cryoelectron Microscopy
- Hemagglutinins
- Receptor, Insulin
- Water