Efficient C-to-T base editing in plants using a fusion of nCas9 and human APOBEC3A.
basic_science · Level V
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- Record sourced from PubMed, PMID 30272679.
- Also identified by DOI 10.1038/nbt.4261.
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Abstract
Base editors (BEs) have been used to create C-to-T substitutions in various organisms. However, editing with rat APOBEC1-based BE3 is limited to a 5-nt sequence editing window and is inefficient in GC contexts. Here, we show that a base editor fusion protein composed of Cas9 nickase and human APOBEC3A (A3A-PBE) converts cytidine to thymidine efficiently in wheat, rice and potato with a 17-nucleotide editing window at all examined sites, independent of sequence context.