Robust Activity of Avapritinib, Potent and Highly Selective Inhibitor of Mutated KIT, in Patient-derived Xenograft Models of Gastrointestinal Stromal Tumors.

Abstract

Gastrointestinal stromal tumors (GIST) are commonly treated with tyrosine kinase inhibitors (TKI). The majority of patients with advanced GIST ultimately become resistant to TKI due to acquisition of secondary <i>KIT</i> mutations, whereas primary resistance is mainly caused by <i>PDGFR</i>A p.D842V mutation. We tested the activity of avapritinib, a potent and highly selective inhibitor of mutated KIT and PDGFRA, in three patient-derived xenograft (PDX) GIST models carrying different <i>KIT</i> mutations, with differential sensitivity to standard TKI.<b>Experimental Design:</b> NMRI <i>nu/nu</i> mice (<i>n</i> = 93) were transplanted with human GIST xenografts with <i>KIT</i> exon 11+17 (UZLX-GIST9 <i>^{KIT 11+17}</i> ), exon 11 (UZLX-GIST3 <i>^{KIT 11}</i> ), or exon 9 (UZLX-GIST2B <i>^KIT9</i> ) mutations, respectively. We compared avapritinib (10 and 30 mg/kg/once daily) versus vehicle, imatinib (50 mg/kg/bid) or regorafenib (30 mg/kg/once daily; UZLX-GIST9 <i>^KIT11+17</i> ); avapritinib (10, 30, 100 mg/kg/once daily) versus vehicle or imatinib [UZLX-GIST3 <i>^KIT11</i> ]; and avapritinib (10, 30, 60 mg/kg/once daily) versus vehicle, imatinib (50, 100 mg/kg/twice daily), or sunitinib (40 mg/kg/once daily; UZLX-GIST2B <i>^KIT9</i> ). In all models, avapritinib resulted in reduction of tumor volume, significant inhibition of proliferation, and reduced KIT signaling. In two models, avapritinib led to remarkable histologic responses, increase in apoptosis, and inhibition of MAPK-phosphorylation. Avapritinib showed superior (UZLX-GIST9 <i>^{KIT 11+17}</i> and -GIST2B <i>^{KIT 9}</i> ) or equal (UZLX-GIST3 <i>^{KIT 11}</i> ) antitumor activity to the standard dose of imatinib. In UZLX-GIST9 <i>^{KIT 11+17}</i> , the antitumor effects of avapritinib were significantly better than with imatinib or regorafenib. Avapritinib has significant antitumor activity in GIST PDX models characterized by different <i>KIT</i> mutations and sensitivity to established TKI. These data provide strong support for the ongoing clinical trials with avapritinib in patients with GIST (NCT02508532, NCT03465722).