Multicolor single-particle reconstruction of protein complexes.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 30275574.
- Also identified by DOI 10.1038/s41592-018-0140-x and PMC identifier 6173288.
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Abstract
Single-particle reconstruction (SPR) from electron microscopy (EM) images is widely used in structural biology, but it lacks direct information on protein identity. To address this limitation, we developed a computational and analytical framework that reconstructs and coaligns multiple proteins from 2D super-resolution fluorescence images. To demonstrate our method, we generated multicolor 3D reconstructions of several proteins within the human centriole, which revealed their relative locations, dimensions and orientations.
Medical subject headings
- Centrioles
- Image Processing, Computer-Assisted
- Microscopy, Electron
- Multiprotein Complexes
- Single Molecule Imaging