Comprehensive identification of peptides in tandem mass spectra using an efficient open search engine.
basic_science · Level V
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- Record sourced from PubMed, PMID 30295672.
- Also identified by DOI 10.1038/nbt.4236.
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Abstract
We present a sequence-tag-based search engine, Open-pFind, to identify peptides in an ultra-large search space that includes coeluting peptides, unexpected modifications and digestions. Our method detects peptides with higher precision and speed than seven other search engines. Open-pFind identified 70-85% of the tandem mass spectra in four large-scale datasets and 14,064 proteins, each supported by at least two protein-unique peptides, in a human proteome dataset.