DNA engineered micromotors powered by metal nanoparticles for motion based cellphone diagnostics.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 30327456.
- Also identified by DOI 10.1038/s41467-018-06727-8 and PMC identifier 6191441.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
HIV-1 infection is a major health threat in both developed and developing countries. The integration of mobile health approaches and bioengineered catalytic motors can allow the development of sensitive and portable technologies for HIV-1 management. Here, we report a platform that integrates cellphone-based optical sensing, loop-mediated isothermal DNA amplification and micromotor motion for molecular detection of HIV-1. The presence of HIV-1 RNA in a sample results in the formation of large-sized amplicons that reduce the motion of motors. The change in the motors motion can be accurately measured using a cellphone system as the biomarker for target nucleic acid detection. The presented platform allows the qualitative detection of HIV-1 (n = 54) with 99.1% specificity and 94.6% sensitivity at a clinically relevant threshold value of 1000 virus particles/ml. The cellphone system has the potential to enable the development of rapid and low-cost diagnostics for viruses and other infectious diseases.
Medical subject headings
- Cell Phone
- HIV Infections
- HIV-1
- Metal Nanoparticles
- Nucleic Acid Amplification Techniques